Journal: Journal of Clinical Microbiology
Article Title: Characterization of Multiple Cytomegalovirus Drug Resistance Mutations Detected in a Hematopoietic Stem Cell Transplant Recipient by Recombinant Phenotyping
doi: 10.1128/JCM.02205-14
Figure Lengend Snippet: Replicative capacities of wild-type and recombinant viruses harboring mutations in the UL97 (A) and UL54 and/or UL97 (B) genes determined by the Gaussia luciferase (GLuc) reporter-based assay over time. Human foreskin fibroblasts were infected with the different recombinant viruses at a multiplicity of infection of 0.002, and the GLuc activities were measured in cell culture supernatants sampled daily for 7 days. Results represent the means ± standard deviation of sextuplicate determinations of one representative experiment. RLU, relative light unit. *, P < 0.05, and ***, P < 0.001, compared with wild type by using a two-way analysis of variance (ANOVA) test (GraphPad Prism version 5.00).
Article Snippet: *, P < 0.05, and ***, P < 0.001, compared with wild type by using a two-way analysis of variance (ANOVA) test (GraphPad Prism version 5.00).
Techniques: Recombinant, Luciferase, Reporter Assay, Infection, Cell Culture, Standard Deviation